mgmt gene promoter methylation assays  (NeoGenomics)

Journal: JAMA Oncology

Article Title: Association of Autologous Tumor Lysate-Loaded Dendritic Cell Vaccination With Extension of Survival Among Patients With Newly Diagnosed and Recurrent Glioblastoma

doi: 10.1001/jamaoncol.2022.5370

Figure Lengend Snippet: A, Kaplan-Meier plot comparing overall survival for patients with newly diagnosed glioblastoma treated with autologous tumor lysate-loaded dendritic cell vaccination (DCVax-L) and 1366 contemporaneous matched external control participants (ECPs) treated with standard of care, derived from 5 other contemporaneous matched randomized clinical trials. B, Cox hazard ratios of overall survival in prespecified subgroups of participants receiving DCVax-L or treated with standard of care in external trials. In the age subgroup, there were 50 participants in the DCVax-L group and 45 in the ECP group aged 65 years or greater and 182 and 184, respectively in the younger than 65 years group; in the residual disease subgroup, there were 86 patients in the DCVax-L group and 163 in the ECP group with significant residual disease and 146 and 210, respectively, with minimal residual disease; in the MGMT (O 6 -methylguanine-DNA methyltransferase) subgroup, there were 90 patients in the DCVax-L group and 199 in the ECP group with methylated MGMT and 131 and 349, respectively, with unmethylated MGMT. Subgroup analyses of survival, using the same parameters as the comparator publications, are presented with 95% confidence intervals to facilitate comparisons with the ECP.

Article Snippet: The MGMT (O 6 -methylguanine-DNA methyltransferase) gene promoter methylation status, IDH (isocitrate dehydrogenase) R132 mutation status, and postsurgery minimal (<2 cm 2 ) vs significant (≥2 cm 2 ) residual tumor were determined centrally (LabCorp; Mayo; ICON).

Techniques: Control, Derivative Assay, Clinical Proteomics, Methylation

Journal: Neuro-oncology Advances

Article Title: Prognostic value of O 6 -methylguanine-DNA methyltransferase methylation in isocitrate dehydrogenase mutant gliomas

doi: 10.1093/noajnl/vdac030

Figure Lengend Snippet: Summary of Cohort Characteristics and Treatments

Article Snippet: The majority of MGMT gene promoter methylation assays was performed by LabCorp or NeoGenomics Laboratories using bisulfite modification of tumor deoxyribonucleic acid (DNA) and polymerase chain reaction (PCR) to detect CpG methylation.

Techniques: Methylation, Biomarker Discovery

Journal: Neuro-oncology Advances

Article Title: Prognostic value of O 6 -methylguanine-DNA methyltransferase methylation in isocitrate dehydrogenase mutant gliomas

doi: 10.1093/noajnl/vdac030

Figure Lengend Snippet: Overall survival (OS) outcomes of patients stratified by MGMT promoter methylation status. (A) OS of the entire patient cohort. (B) OS of patients diagnosed with glioblastoma multiforme (GBM). (C) OS of patients diagnosed with anaplastic astrocytoma (AA) and low-grade astrocytoma (LA). (D) OS of patients diagnosed with anaplastic oligodendroglioma (AO) and low-grade oligodendroglioma (LO). (E) OS of patients diagnosed with AA. (F) OS of patients diagnosed with LA. (G) OS of patients diagnosed with AO. (H) OS of patients diagnosed with LO.

Article Snippet: The majority of MGMT gene promoter methylation assays was performed by LabCorp or NeoGenomics Laboratories using bisulfite modification of tumor deoxyribonucleic acid (DNA) and polymerase chain reaction (PCR) to detect CpG methylation.

Techniques: Methylation

Journal: Neuro-oncology Advances

Article Title: Prognostic value of O 6 -methylguanine-DNA methyltransferase methylation in isocitrate dehydrogenase mutant gliomas

doi: 10.1093/noajnl/vdac030

Figure Lengend Snippet: Multivariate Analysis of OS in Various Pathological Subgroups

Article Snippet: The majority of MGMT gene promoter methylation assays was performed by LabCorp or NeoGenomics Laboratories using bisulfite modification of tumor deoxyribonucleic acid (DNA) and polymerase chain reaction (PCR) to detect CpG methylation.

Techniques:

Journal: Neuro-oncology Advances

Article Title: Prognostic value of O 6 -methylguanine-DNA methyltransferase methylation in isocitrate dehydrogenase mutant gliomas

doi: 10.1093/noajnl/vdac030

Figure Lengend Snippet: Progression-free survival (PFS) outcomes of patients stratified by MGMT promoter methylation status. (A) PFS of the entire patient cohort. (B) PFS of patients diagnosed with glioblastoma multiforme (GBM). (C) PFS of patients diagnosed with anaplastic astrocytoma (AA) and low-grade astrocytoma (LA). (D) PFS of patients diagnosed with anaplastic oligodendroglioma (AO) and low-grade oligodendroglioma (LO). (E) PFS of patients diagnosed with AA. (F) PFS of patients diagnosed with LA. (G) PFS of patients diagnosed with AO. (H) PFS of patients diagnosed with LO.

Article Snippet: The majority of MGMT gene promoter methylation assays was performed by LabCorp or NeoGenomics Laboratories using bisulfite modification of tumor deoxyribonucleic acid (DNA) and polymerase chain reaction (PCR) to detect CpG methylation.

Techniques: Methylation

Journal: Neuro-oncology Advances

Article Title: Prognostic value of O 6 -methylguanine-DNA methyltransferase methylation in isocitrate dehydrogenase mutant gliomas

doi: 10.1093/noajnl/vdac030

Figure Lengend Snippet: Multivariate Analysis of PFS in Various Pathological Subgroups

Article Snippet: The majority of MGMT gene promoter methylation assays was performed by LabCorp or NeoGenomics Laboratories using bisulfite modification of tumor deoxyribonucleic acid (DNA) and polymerase chain reaction (PCR) to detect CpG methylation.

Techniques:

Journal: Neuro-oncology Advances

Article Title: Prognostic value of O 6 -methylguanine-DNA methyltransferase methylation in isocitrate dehydrogenase mutant gliomas

doi: 10.1093/noajnl/vdac030

Figure Lengend Snippet: Summary of Cohort Characteristics and Treatments

Article Snippet: The majority of MGMT gene promoter methylation assays was performed by LabCorp or NeoGenomics Laboratories using bisulfite modification of tumor deoxyribonucleic acid (DNA) and polymerase chain reaction (PCR) to detect CpG methylation.

Techniques: Methylation, Biomarker Discovery

Journal: Neuro-oncology Advances

Article Title: Prognostic value of O 6 -methylguanine-DNA methyltransferase methylation in isocitrate dehydrogenase mutant gliomas

doi: 10.1093/noajnl/vdac030

Figure Lengend Snippet: Overall survival (OS) outcomes of patients stratified by MGMT promoter methylation status. (A) OS of the entire patient cohort. (B) OS of patients diagnosed with glioblastoma multiforme (GBM). (C) OS of patients diagnosed with anaplastic astrocytoma (AA) and low-grade astrocytoma (LA). (D) OS of patients diagnosed with anaplastic oligodendroglioma (AO) and low-grade oligodendroglioma (LO). (E) OS of patients diagnosed with AA. (F) OS of patients diagnosed with LA. (G) OS of patients diagnosed with AO. (H) OS of patients diagnosed with LO.

Article Snippet: The majority of MGMT gene promoter methylation assays was performed by LabCorp or NeoGenomics Laboratories using bisulfite modification of tumor deoxyribonucleic acid (DNA) and polymerase chain reaction (PCR) to detect CpG methylation.

Techniques: Methylation

Journal: Neuro-oncology Advances

Article Title: Prognostic value of O 6 -methylguanine-DNA methyltransferase methylation in isocitrate dehydrogenase mutant gliomas

doi: 10.1093/noajnl/vdac030

Figure Lengend Snippet: Multivariate Analysis of OS in Various Pathological Subgroups

Article Snippet: The majority of MGMT gene promoter methylation assays was performed by LabCorp or NeoGenomics Laboratories using bisulfite modification of tumor deoxyribonucleic acid (DNA) and polymerase chain reaction (PCR) to detect CpG methylation.

Techniques:

Journal: Neuro-oncology Advances

Article Title: Prognostic value of O 6 -methylguanine-DNA methyltransferase methylation in isocitrate dehydrogenase mutant gliomas

doi: 10.1093/noajnl/vdac030

Figure Lengend Snippet: Progression-free survival (PFS) outcomes of patients stratified by MGMT promoter methylation status. (A) PFS of the entire patient cohort. (B) PFS of patients diagnosed with glioblastoma multiforme (GBM). (C) PFS of patients diagnosed with anaplastic astrocytoma (AA) and low-grade astrocytoma (LA). (D) PFS of patients diagnosed with anaplastic oligodendroglioma (AO) and low-grade oligodendroglioma (LO). (E) PFS of patients diagnosed with AA. (F) PFS of patients diagnosed with LA. (G) PFS of patients diagnosed with AO. (H) PFS of patients diagnosed with LO.

Article Snippet: The majority of MGMT gene promoter methylation assays was performed by LabCorp or NeoGenomics Laboratories using bisulfite modification of tumor deoxyribonucleic acid (DNA) and polymerase chain reaction (PCR) to detect CpG methylation.

Techniques: Methylation

Journal: Neuro-oncology Advances

Article Title: Prognostic value of O 6 -methylguanine-DNA methyltransferase methylation in isocitrate dehydrogenase mutant gliomas

doi: 10.1093/noajnl/vdac030

Figure Lengend Snippet: Multivariate Analysis of PFS in Various Pathological Subgroups

Article Snippet: The majority of MGMT gene promoter methylation assays was performed by LabCorp or NeoGenomics Laboratories using bisulfite modification of tumor deoxyribonucleic acid (DNA) and polymerase chain reaction (PCR) to detect CpG methylation.

Techniques:

Journal: Oncology Letters

Article Title: Effects of valproic acid on the susceptibility of human glioma stem cells for TMZ and ACNU

doi: 10.3892/ol.2018.8551

Figure Lengend Snippet: Methylation-specific amplification primers for the MGMT promoter.

Article Snippet: Following treatment of samples with methylation-specific PCR (MSP), as described previously , MGMT gene promoter methylation amplification primers were designed in Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA) and are summarized in .

Techniques: Methylation, Amplification, Sequencing

Journal: Oncology Letters

Article Title: Effects of valproic acid on the susceptibility of human glioma stem cells for TMZ and ACNU

doi: 10.3892/ol.2018.8551

Figure Lengend Snippet: Expression of O6-methylguanine-DNA methyltransferase protein in 3 glioma stem cell populations. (A) G1 cell population. (B) G2 cell population. (C) G3 cell population. Brown-colored particles in the nucleus or cytoplasm indicated the presence of MGMT-positive cells. Magnification, ×400.

Article Snippet: Following treatment of samples with methylation-specific PCR (MSP), as described previously , MGMT gene promoter methylation amplification primers were designed in Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA) and are summarized in .

Techniques: Expressing

Journal: Oncology Letters

Article Title: Effects of valproic acid on the susceptibility of human glioma stem cells for TMZ and ACNU

doi: 10.3892/ol.2018.8551

Figure Lengend Snippet: Changes in MGMT expression in glioma stem cells. The expression of MGMT in the (A-a1) G1, (B-b1) G2 and (C-c1) G3 cell populations cultured in VPA-free medium, and MGMT expression in the (A-a2) G1, (B-b2) G2 and (C-c2) G3 cells populations exposed to 1 mmol/l VPA for 3 days. MGMT, O6-methylguanine-DNA methyltransferase; VPA, valproic acid.

Article Snippet: Following treatment of samples with methylation-specific PCR (MSP), as described previously , MGMT gene promoter methylation amplification primers were designed in Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA) and are summarized in .

Techniques: Expressing, Cell Culture

Journal: Oncology Letters

Article Title: Effects of valproic acid on the susceptibility of human glioma stem cells for TMZ and ACNU

doi: 10.3892/ol.2018.8551

Figure Lengend Snippet: Changes in MGMT promoter methylation. There was partial methylation of G1 cell line in the control group, while complete methylation following VPA treatment, and non-methylation in the G2 and G3 cell populations in the control group, but partial methylation following VPA treatment. U, non-methylated primer amplification; M, the methylation primer amplification; VPA, valproic acid.

Article Snippet: Following treatment of samples with methylation-specific PCR (MSP), as described previously , MGMT gene promoter methylation amplification primers were designed in Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA) and are summarized in .

Techniques: Methylation, Control, Amplification